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How to Isolate Bacterial Colonies Using the Streak Plate Method May 20, 2014 · Step 3: Sterilize your loop using a Bunsen burner or a “cinerator” such as this. Allow the loop to cool (without setting it down) for several seconds. Then, drag the loop gently through your first quadrant (only once.) Continue to streak the second quadrant of your plate in a similar zig-zag manner from the outer edge of the plate inward. Bunsen Burner Labeled Diagram Stock Vector - Dreamstime Illustration about a bunsen burner used in a science lab. Illustration of science, problems, label - 42220489. BAM Chapter 14: Bacillus cereus | FDA WebHeat slide gently from below with small Bunsen burner until steam is seen. Wait 1-2 min and repeat this step. Let stand 30 s, pour off stain, and rinse slide thoroughly with clean tap water. Bunsen burner label